Real-time monitoring was possible by utilizing a DNA-specific fluorescent dye and introducing a denaturation step for the paired oligonucleotides, at the highest possible temperature, where the LCR amplicons remained as heterodimers.
Transcriptional control of a gene is often studied by utilizing a fusion of the gene's promoter with a reporter gene [chloramphenicol acetyRransferase (CAT), green fluorescent protein (GFP), and so on].
Unlike the experiments conducted by Schrader and group's, we utilized a fluorescent probe rather than a chemiluminescence probe lucigenin.
The assay design utilizes a fluorescent phosphorylated peptide complexed to a product-specific anti-phospho-serine antibody.
To further characterize Ltbp1L activity within the luminal lineage we utilized a fluorescent β-Galactosidase substrate, FDG-Gal to detect Ltbp1L-positive cells by flow cytometry.
In a previous in vitro study with recombinant αBwt, αAwt and the C-terminal truncated αA-crystallins and by utilizing fluorescent chemical probes in fluorescence resonance energy transfer (FRET) analysis, we have observed C-terminal truncation affecting interaction with αAwt and αBwt [18].
However, one group based in the United Kingdom attempted to resolve telomere length at the level of the individual chromosome by utilizing semiquantitative fluorescent in situ hybridization (QFISH) with individual gametes and pronuclear embryos [ 41, 42].
Immunofluorescence staining was performed by utilizing red fluorescent anti-sheep or anti-rabbit IgG (Alex 594, Invitrogen) for one hour, then cover slips were washed three times with PBS (10 min/each time).
In this study, by utilizing live cells fluorescent microscopy, P-ICP22 fused to enhanced yellow fluorescent protein (EYFP) gene was transient expressed in live cells and shown to exhibit a predominantly nucleus localization in the absence of other viral proteins.
Alternative optical methods for this task have been demonstrated promising by utilizing fluorescent labeling of cells flowing in the blood stream using a non-imaging modality [ 4] and at cellular resolution [ 5].
