We herein reported that Hedgehog signaling is one of the causative factors for omphalocele formation, as demonstrated by utilizing a series of combinatorial mutants for Hh signaling genes and conditional gain-of-function mutants of the Hh signaling pathway.
Recent advancements in the production of isoprenoids, phenylpropanoids, and alkaloids were made possible by utilizing a variety of techniques including combinatorial biosynthesis, codon optimization, expression of regulatory elements, and protein engineering of P450s.
Physicochemical property mining module (PCP mining module) solved this optimization problem by utilizing an inheritable bi-objective combinatorial optimization genetic algorithm (IBCGA).
In the current study, we utilized a combinatorial approach of cell culture and in vivo biopanning using an M13 phage peptide display library to identify peptide(s) with potential for cardiomyocyte transduction in vivo in a tissue specific manner.
For instance, it is known that the transcription regulation of genes is much more intricate in eukaryotes, which utilize a combinatorial coding of different transcription factors [ 13, 14].
To gain global insights into genes, transcription factors and kinases regulated by these stresses and to explore information on coding transcripts that are associated with traits of agronomic importance, we utilized a combinatorial approach of next generation sequencing and de-novo assembly to discover B. juncea transcriptome associated with high temperature and drought stresses.
To respond to the challenge, probe technology is being revolutionized by utilizing methods of combinatorial chemistry, phage display and directed molecular evolution.
Utilizing a newly designed combinatorial histone peptide microarray, we showed that three reader domains (ATRX-ADD, ING2-PHD and AIRE-PHD) displayed greater specificity towards combinatorial PTM patterns than corresponding commercial histone antibodies.
For this purpose, we proposed a novel data assimilation algorithm utilizing a simple nonlinear model, termed the combinatorial transcription model [ 5, 30], and a state space representation [ 31, 32], to infer GRNs by restoring networks that is inferred by some GRNs inference methods or derived from the literature.
As an initial step of incorporating post-translational modifications into combinatorial libraries, we present a bacterial co-expression system, utilizing a recently characterized calmodulin methyltransferase (CaM KMT), to trimethylate a combinatorial library of the calmodulin central linker region.
