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Living and fixed cells were imaged by using widefield microscopy as previously described [56].
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This technique can be employed to separate single active fibers within complex neuronal circuits from background information, even using widefield microscopy.
SIM provides a twofold increase in this resolution limit (Gustafsson et al., 2008), and we were able to detect two foci of Spc42-GFP that were unresolvable using widefield microscopy.
In silico microangiography is a simple and non-invasive technique that uses digital image processing of images obtained using widefield microscopy and a CCD camera, and delivers results similar to laborious dye injection-based microangiography methods [ 15].
For BiFC assays, the pYFN43::SPL7 (nYFP::SPL7) and pYFC43::SPL7 (cYFP::SPL7) destiny vectors were individually or co-expressed in leaf epidermal tobacco cells as indicated above, and examined using widefield epifluorescence microscopy (Olympus BX61) and confocal laser scanning microscopy to asses the restoration of the YFP signal.
The antibacterial effect was evaluated using widefield fluorescence microscopy.
The DAPI images were collected using widefield fluorescence and deconvolution software.
Correct labeling of the two proteins was confirmed using widefield imaging.
Infection efficiencies were evaluated by using confocal microscopy analysis.
Cellular morphological changes were examined by using fluorescence microscopy (×100).
The fleas were identified by using light microscopy.
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