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Dead cells were eliminated by using viability dye 7-AAD.
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In addition, by using cell viability assay, the researchers showed that differentiated cells were more resistant to exogenous ROS stress than undifferentiated cells.
Dead cells were excluded by using Fixable Viability Dye (eBioscience).
Inactivation was measured by using a viability dye (methylene blue) exclusion method where uptake of dye indicates cell death and inactivation.
We next measured the accuracy of yeast cell viability by using 0%, 25 %, 50 %, 75 % and 100iability samples.
Cells viability was assessed at each time point by using PrestoBlue™ Viability Reagent (life technologies ref: A13262) according to the manufacturer's instructions.
Compared to 1 (steroidal aromatase inactivator), the transformed metabolites were also evaluated for cytotoxic activity by using a cell viability assay against cancer cell lines (HeLa and PC3).
Since this imply that AgNP toxicity in addition to altering BPE will also induce cell death, we verified if cell destruction by AgNP happens in our case by using MTT cell viability assay.
With the proposed method, we measured the precision of yeast cell measurements by using 0%, 25 %, 50 %, 75 % and 100iability samples.
Cytotoxicity of Gd DB-HNTs was evaluated by usinGd DB-HNTsiability assay (MTT, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Sigma-Aldrich, St. Louis, MO))).
In addition to the qualitative assessment, we also quantified the relative percentage of adherent cells remaining after GzmB treatment by using a MTS viability assay.
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