By using various sequencing platforms, 6.7X, 10X, and 28X physical coverage of whole genome sequence of N. bombycis were obtained from the Sanger sequencing method (plasmids with 2Kb inserts), the miniBAC end sequencing method, and the Illumina short-read sequencing method respectively (Additional file 1).
We used various sequence and chromatin data to compute feature values at SEs and TEs (Methods).
We also use various sequence- and structure-based bioinformatics approaches to study cadherin diversity and evolution.
This analysis resulted in more sequence contigs than generated by previous studies which used various sequencing technologies, though the average length of our contigs was shorter.
The miRanda algorithm computes an optimal local alignment of the miRNA with an mRNA sequence, by using various parameters for the overall alignment score, gap opening, and gap extension penalties.
Notably, by using various nuclear import or export sequences fused with VHL, a previous study has indirectly showed that specific subcellular localization affects the antitumor properties of VHL [28].
Discrete Applied Mathematics 2001;110 13 24] and best next for maximal available symbol algorithm [Huang KS, Yang CB, Tseng KT. Fast algorithms for finding the common subsequence of multiple sequences. In: Proceedings of international computer symposium; 2004. p. 90 95] were tested and compared by using various sets of DNA and protein sequences.
Without a well assembled whole genome sequences, comparative genome analysis can be achieved by using various other genomic resources containing information for genome level conservation.
Many pufM sequences were related to the purple non-sulfur bacteria; these bacteria preferentially grow photoheterotrophically under anaerobic conditions in the light by using various organic substrates.
Partial human GPR147 cDNA was amplified by PCR using various primers based on human GPR147 cDNA sequence (NM_022146).
