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The biocompatibility effect of as-prepared AuNPs was investigated in MDA-MB-231 breast cancer cells by using various concentrations of AuNPs (10 to 100 μM) for 24 h.
The same procedure was adopted by using various concentrations of the examined compounds (0 1 mM final concentration), and then the following equation was applied using a linear regression analysis by plotting 1/A vs [S].
Unless otherwise indicated, enzymatic activity was assayed at 37°C by using various concentrations of R5P and Mg-ATP under conditions identical to those described above except for substrates and effectors.
To determine whether PL affects the invasive ability of human colon cancer cells in vitro, we performed invasion and motility assays by using various concentrations of PL.
Experiments were then carried out to determine the maximum rate of catalysis (Vmax) of PDE4D5-WT or PDE4D5-L33D, and the affinity of PDE4D5-WT or PDE4D5-L33D for cAMP (Km) by using various concentrations of substrate (between 1 μM and 40 μM).
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The free thiol concentration of samples was calculated based on the standard curve prepared by using various concentration of L-cysteine.
The mode of inhibition and inhibition parameters, i.e. the Michaelis Menten constant (Km), maximal velocity (Vmax), turnover number (kcat) of TyrBm and the inhibition constants (KI, KIS) of each inhibitor were determined by Lineweaver Burk plot analysis using various concentrations of L-tyrosine (0.03 1.4 mM) and L-dopa (0.15 2.0 mM) as substrates.
New formulation of buccal films containing rizatriptan benzoate (RB) was prepared by solvent casting method using various concentrations of hydroxypropyl methylcellulose (HPMC K4M), polyvinyl alcohol (PVA), polyethylene oxide (PEO), glycerol, stevia, and goat buccal mucosa used as a model membrane.
LaF3 Tb nanoparticles assessed in 3T3 cells were determined using various concentrations by use of the WST-1 assay.
Km was determined for KLK5 using various concentrations of substrate.
Various concentrations of MiTI were assayed in order to determine the mechanism and inhibition constant towards trypsin like midgut proteinases by using various BApNA concentrations as substrate according to Singh et al. [ 13].
More suggestions(17)
by using various values
by spiking various concentrations
by using various polymers
by using various types
by incubating various concentrations
by evaluating various concentrations
by introducing various concentrations
by using various resins
by taking various concentrations
by using various amounts
by doping various concentrations
by using various strategies
by using controlled concentrations
by using various tools
by mixing various concentrations
by using equimolar concentrations
by using proper concentrations
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