Sentence examples for by using universal primers from inspiring English sources

The 16S rRNA gene fragment was amplified by using universal primers corresponding to positions 8 to 27 for the forward primer and 1492 to 1510 for the reverse primer mentioned below (Table 1).

Since ITS1 and ITS2 regions can be amplified by using universal primers, and the results are reliable, rDNA ITS sequence analysis was used in our study to understand the phylogenetic relationship of selected Taiwan-specific Gentiana spp. (GDF, GA and GSP) with Chinese Longdan.

The 16S rRNA gene was amplified by using universal primers 27F and 1492R.

The only positive results were observed by using universal primers for amplification of influenza A viruses.

Respiratory and fecal samples from both centers were screened by using universal primers, and positive samples were digested with RsaI.

Two-step RT-PCR was performed by using universal primers and specific primers for influenza A viruses (21 ).

b distinct FITC colour of stained colonies Further, the 16S rRNA genes of the isolates were amplified by PCR using universal primers [ 7] and subsequently analysed by sequencing.

Segments from these genes can be easily amplified by PCR using universal primers and sequenced.

To confirm the identification of strain SK.DU.4, the 16S rRNA gene was amplified by PCR using universal primers and the amplified PCR product was sequenced as described earlier (Suresh et al. 2006).

Positive clones were screened by PCR using universal primers and sequenced.

Ninety-five samples were analyzed by PCR using universal primers to the 16S rDNA gene.