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Using this method, we conveniently detected polyubiquitin chain formation of E2 and E3s by using two tagged ubiquitins (Fig. 1, 2, 3 and 4).

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We initiated these studies by using two different, N-terminal epitope-tagged versions of Sox2: Flag-Sox2 and GFP-Sox2, which we had previously shown do not adversely affect the function of Sox2 [23] [23].

Autophagy and lysosome activity was also studied using two different transgenic lines of the GFP-tagged Atg8a (GFP:Atg8a) with similar results.

Selective conjugation of azide-tagged cross-linked peptides has been demonstrated using two strategies: copper catalyzed cycloaddition and Staudinger ligation.

We next performed a pull-down assay in which we used two GST-tagged β-catenin fusion proteins.

To test this possibility, we used two different IC-tagged proteins, GFP-IC [11] and p53-IC.

A study used two million geo-tagged photos from Flickr.

The six short PCR products were added to the sheared products, and each pool was tagged with a unique DNA barcode by using the Parallel Tagged Sequencing (PTS) protocol [ 24], starting at the blunt-end repair step.

By using the two heat-shock inducible Wnt inhibition transgenic zebrafish lines, hs:Dkk1-GFP and hs:ΔTcf-GFP, we demonstrated that the expression of GFP-tagged Dkk1/Tcf was induced in the swimbladder.

This was confirmed by using luciferase-tagged PbA and PyYM.

We used four different His-tagged proteins to study the effect of various radiolabelling conditions.

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