Sentence examples for by using two specific from inspiring English sources

The second cost measure is the changeover cost, in which the cost of traversing a vertex by using two specific incident edges is paid only once regardless of the number of paths traversing it.

Subtraction was applied to remove the abundant 28S and 18S ribosomal RNAs by using two specific probes for both 18S ribosomal RNA and 28S ribosomal RNA.

Since the expression of Lif in cultured Muller cells was robust, we first tested the effect of p38 MAPK activity on basal Lif expression in the absence of TNF by using two specific chemical inhibitors for p38 MAPK activity, SB239063 and SB202190.

By using one specific primer and one reverse primer during PCR, the precise sequence of the end of the miRNA opposite to the specific primer could be correctly amplified and validated.

The rDNA was also amplified by using four specific primers (two forward and two reverse) at two different temperatures to obtain a large quantity of DNA for posterior sequencing.

We then examined the cellular localization of ARPC1A and ARPC1B by immunofluorescence using two specific antibodies from different species that allowed simultaneous labelling in the same cells (Supplementary information, Fig. S7).

Discrimination was also possible by parallel testing using two specific mAbs.

The completed ORF (open reading frame) was amplified by nested PCR, using two specific 3' UTR primers and a forward primer from the beginning of the CDS.

We further analyzed the mitochondria involvement in the cell death induced by anti-GD2 mAb using two specific sensitive fluorescent probes JC-1 and DiOC6(3).

Finally the strong phenotype observed in Ci-E z) morphants was Ci-E zterized further by in situ hydridization using two specific morphantsespectively of notochord and striated muscle, namely probes specific of Fibrinogen-like (Fibrn) (Takahashi et al., 1999) and Myosin Regulatory Light Chain 2 (MRLC2) (Ikuta et al., 2010).

Target sites in leptin gene (first 94 bp fragment of exon 2 and 330 bp fragment including first part of exon 3) were amplified by polymerase chain reaction (PCR) using two specific primers pairs.