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The evolution of network convergence can be divided into four stages: Nicholas Negroponte (1973) [1] described the marginal overlay phenomenon of the computer industry, radio and movie industry, and the printing and publishing industry by using three overlapping circles.
mtDNA was enriched from total DNA extract by using three overlapping long-range PCR (LR-PCR) fragments, designed to avoid the amplification of nuclear mitochondrial pseudogenes.
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Pol-amplified products (containing the entire protease and the first 335 amino acids of the reverse transcriptase open reading frame, 1302 nt) were full-length sequenced in sense and antisense orientations by an automated sequencer (ABI 3100) by using seven different overlapping sequence-specific primers [ 21].
Channel reservations have been achieved in [11] by using two ZigBee channels overlapping with the WiFi one: the first channel is adapted to transmit a busy tone able to activate the WiFi carrier sense, while the second channel is used by the other ZigBee nodes to transmit their data in parallel to the busy tone transmission (i.e., while WiFi stations are prevented from accessing the channel).
Sequencing was carried out by using five pairs of overlapping SLEV primers together with T7 and SP6 primers.
We amplified Helitron Hel1-333 from inbred lines HP301, OH7B, and Tzi8 by using two pairs of overlapping primers.
Cellular immune responses were measured by using five complete Env overlapping peptide sets: subtype A (92UG37_A), subtype B (MN_B, 89.6_B and SF162_B), and subtype C (Chn19_C).
Bi-directional PAP-A (Bi-PAP-A) was developed to eliminate the bypass reaction, and thereby increase analytical selectivity, by using two blocked primers that overlap at one base [8], [9] (Fig. S1B).
By using two laser beams, one activates the writing and the second, ring-shaped beam overlaps it.
To overcome difficulties associated with the overlap, the OH and fuel fluorescence signals were partially separated by using two cameras with differing spectral filters and data acquisition timing.
A long-range restriction map of this region was created using eight nonchimeric, overlapping YAC clones.
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