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By using three 15-mer DNA fragments as proof-of-principle analytes, the proposed method showed good analytical performance.
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XIST RNA-FISH was performed as described [38] by using three 50-mer DNA probes designed from consensus sequences of map positions 6183-6232, 62234-6283 and 6368-6417 (accessioNo.o. L04961), which are in repeat D of XIST.
SIV peptide pools each contained ten 15-mer peptides overlapping by eleven amino acids.
By including at least three 60-mer probes per gene, the few 60-mers that underperformed were compensated by flanking 60-mers, and these were randomly distributed.
The DNA sequences were generated by using the 8-mer frequencies observed in mouse genome.
On the array, 0.56% of the genes are covered by one, 14.76% by two, 9.17% by three, 11.15% by four and 63.35% by five 60-mer oligonucleotides.
In this study, we investigated gene expression variations of sweetpotato root at seven different developmental stages by using a customized 60-mer oligonucleotide microarray.
Despite the low score obtained using the class I MHC binding prediction we immunized immune competent IDH1 mutated glioma-bearing mice using four short peptides (two 9-mers and two 10-mers).
Let us consider peptide segment LAGNSSLC, represented by two 7-mer peptides: LAGNSSL and AGNSSLC.
Each ORF was represented by seventeen 60-mer oligonucleotides.
Gap-DNA was assembled by annealing the two 12-mer oligonucleotide sequences flanking the central position to the 25-mer opposite strand.
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