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Furthermore, by using this sample, TCRαβ+CD25highEdU+ or EdU– Tregs could be isolated by FACSAria sorting, and Foxp3 message could be detected in both cell groups by RT-PCR (Fig. 3a).
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By using this filtered sample set, we calculated quality control statistics, and SNPs with call rates <98% or SNPs with a Hardy Weinberg equilibrium test P value <1.0E−06 were excluded.
By using this approach, samples from 82626%) of 31 red colobus plasma scored positive (>200 ELISA units [EU]), 21 (68%) of 31 scored seronegative (<100 EU), and 2 (6%) of 31 had equivocal results (100 200 EU) (Appendix Figure).
By using this method, with this sample, we are able to identify a list of priorities where each respondent received equal weighting of input.
By using this method, composite samples were synthetized without the production of any potentially hazardous waste.
However, it should be noted that the optimised region obtained by utilizing the hedonic scale with reference refer to samples whose acceptability are likely to be greater than that of the reference sample, as by using this scale consumers are forced to evaluate each sample in relation to the reference.
Indeed, they could only retrieve their results by using this number since blood samples were not otherwise labeled.
Nonetheless, we reduced the likelihood that possible selection bias influenced the results by using this objective method of sampling renal literature.
By using this system the complexity of sample handling is greatly reduced and the need for making unique primer combinations for each sample is eliminated.
By using this method, only one small sample was needed, therefore self-consistency was easily achieved.
Although physicians per 1,000 capita is a very common metric in estimating health system efficiency, we lost about twenty percent of our sample by using this metric.
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