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The DNA was purified by using the Wizard DNA Purification Kit (Promega, Madison, WI).
Genomic DNA from C. jejuni was prepared as described previously [27] or purified by using the Wizard Genomic DNA kit (Promega, Madison, WI), according to the manufacturer's specifications.
The PCR product was purified by using the Wizard SV Gel and PCR Clean-Up System PROMEGAandand used as template for a second PCR in order to extend the homologous recombination regions.
The different probes were recovered after agarose gel electrophoresis by using the "Wizard SV gel Kit", then labeled with 925 kBq of [α32P] dCTP (110 TBq/mmol) from Amersham Pharmacia Biotech., using the Promega Corp. "Random Primers DNA Labeling Kit".
Between 4 and 6 colonies were selected, and plasmid DNA was prepared from each bacterial culture by using the Wizard Plus plasmid DNA purification kit (Promega, Madison, WI), following the manufacturer's suggested protocol.
Bisulfite-treated DNA was collected by using the Wizard™ DNA Clean-up system.
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Transposon fragments were purified using either the UltraClean GelSpin DNA purification kit (Mol Bio, Carlsbad, CA) or electroelution followed by concentration using the Wizard® DNA Clean-Up System (Promega).
The substructure determination, phasing, density modification and initial automatic model building were all performed by using the AutoSol Wizard of Phenix[44].
Genomic DNA from peripheral blood was extracted by using the Promega wizard genomic DNA isolation kit (http://www.promega.com).
Cell phase analysis was done with ModFit LT 3.2 software by using the Sync Wizard model (30000 cells/sample in biological duplicate).
Genomic DNA was extracted by standard methods using the Wizard Genomic DNA Purification System (Promega).
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