Specifically, we generated Col2-Cre3 R26 Col2-Cre3 R26(termed C2Wnt7b/+ice) by using the Col2-Cre3 transgenic line that targets both osteoblasts and chondrocytes.
Therefore, we next focused on the possibility of introducing LMG 23827T or LMG 23827T::GFP into 2 dpf-old embryos directly by microinjection into the yolk sac, the common cardinal vein or the hindbrain ventricle, thereby exploiting the advantage of easy fluorescent tracking by using the GFP transgenic strain C. turicensis LMG 23827T::GFP with concentrations ranging from 50 to 10 CFUs.
The above observations are corroborated by our findings using the transgenic frogs described here.
We chose to study how the YB and YD sites diverge in their contributions to early stages of metastasis by using the Neu-transgenic mouse model and in vivo assays for tumor cell motility, invasion and intravasation.
To ask whether thioredoxin reductases are essential in brain development and function, we inactivated both Txnrd1 and Txnrd2 individually in the nervous system (NS) by using the Nestin-Cre transgenic mouse [18], which expresses Cre in neuronal and glial precursor cells.
We tested this possibility by using the fus3 FUS3 FUS3-GR transgenic lines.
To further characterize the role of hda-1 in reproductive system development, we examined its expression profile by using the gfp reporter transgenic strains sEx13706 and bhEx72.
First, we probed the overall activity of the larva's visual system from 5 to 7 dpf in response to defined visual stimuli by using the Tg(HuC GCaMP5G) transgenic line, in which among other neuron types, all RGCs and PVNs are labeled (Ahrens et al., 2013).
We then set out to confirm the effect of Notch signaling in macrophages on renal fibrosis by using the myeloid-specific NIC transgenic mice (Zhao et al., 2016) (Fig. S6).
These observations are similar to earlier observations by using the heat-shock inducible transgenic lines to block Wnt signalings (Figs. 4, 5, 6).
