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Serum alanine aminotransferase (ALT and AST) activities were determined by using the serum aminotransferase test kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturer's instructions.
Consequently, many cases of CKD could be missed by using the serum creatinine alone.
HBV DNA was extracted from 200 μl sera by using the serum viral DNA purification kit (PG Biotechnology, Shenzhen, China).
For the purpose of the present RI analysis, renal function was assessed by using the serum creatinine level to calculate creatinine clearance (CLCr) with the Cockroft-Gault equation.
Plasma-neutralizing antibodies against NiV were measured by using the serum neutralization test (SNT) (23 ) with plasma diluted 1 2 1 1,024.
By using the serum enzyme-linked immunosorbent assay, it was found that healthy women had lower serum hepcidin levels than healthy men [ 101].
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By using the serum-induced arthritis model in this study, we were able to pinpoint redox regulation of IL-1β production as the likely cause of increased joint inflammation in ROS-deficient conditions and unravel a potential immune mechanism underlying this unique redox-regulated immune process.
Severity of AKI according to the RIFLE classification was established by using the highest serum creatinine level and/or the lowest urine output.
These results were confirmed by using the CD25KO serum in the first step of the co-precipitation, and bands were correctly identified in the immunoprecipitate by using the commercial antibody (data not shown).
We evaluated these biomarkers' capability of detecting AKI and its severity as determined by using the Kidney Disease Improving Global Outcomes serum creatinine criteria, as well as its capacity to predict in-hospital mortality.
The extracted proteins were separated by SDS-PAGE (10% acrylamide), followed by Western blot using the serum IgE from individual patients, as described previously (Usui et al., [2001]; Hirano et al., [2013]).
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by using the testing
by using the vaccine
by using the blood
by using the sample
by using the drug
by repressing the serum
by inoculating the serum
by extracting the serum
by using the city
by prolonging the serum
by measuring the serum
by using the profile
by clearing the serum
by extrapolating the serum
by using the election
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