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In Mozart's music a harmonic progression from tonic chord (I) to dominant chord (V) will often pass through the dominant of the dominant (V-of-V): from I to V-of-V to V. By using the secondary dominant, he expanded the harmonic range of the composition by introducing chromaticism.
The membrane integrity was checked not only by the tensile strength of the modified membrane samples but also by the long term operation by using the secondary effluent of the wastewater treatment plant as a feed.
The hydrogen adsorption capacity of modified Zn2 bdc 2dabco was significantly enhanced up to 3.35 wt.% by using the secondary spillover with carbon bridges measured at 1.5 bar and 77 K.
The antigen-antibody reaction was developed by using the secondary antibody fluorescein-labeled donkey anti-goat IgG (Rockland, Philadelphia, Pennsylvania) at 1∶250 dilution for 1 h.
An antibody binding was visualised by using the secondary antibodies conjugated with horseradish peroxidase and an AEC kit according to the manufacturers' protocols (Dako Corporation, Denmark).
These results show that the computing speeds are greatly improved by using the secondary development program, while the statistical parameters of models are as good as those obtained with the Gaussian method.
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To test this hypothesis we aligned the 954 residues of mt-12S rRNA and the 1542 residues of E. coli 16S rRNA by using the secondary-structure maps as a reference (not shown).
Detection of antibody binding was performed by using the following secondary reagents: DAKO peroxidase EnVision system for the detection of mouse and rabbit antibodies, and Sigma biotinylated anti-goat antibody (followed by extra avidin peroxidase reagent) for goat antibodies.
Immunodetection was completed by using the corresponding secondary horseradish peroxide- conjugated antibodies (Amersham, Piscataway, NJ).
Our study aimed to assess vaccine-induced reductions in susceptibility and infectiousness among children by using the household secondary attack rate.
Seminal studies already addressed the robustness of RNA molecules (Wagner and Stadler 1999; Ancel and Fontana 2000) by using the predicted secondary structure as a model to link phenotype (structure) and genotype (sequence).
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