Sentence examples for by using the restriction from inspiring English sources

Both ORFs were cloned into the pBSII KS plasmid by using the restriction enzymes NotI and KpnI.

Pulsed-field gel electrophoresis (PFGE) was performed by using the restriction endonucleases SmaI and AscI (5 ).

PFGE was performed as described (12 ) by using the restriction enzyme smaI (New England BioLabs, Ipswich, MA, USA).

Ribotyping was performed by using the restriction enzyme EcoRI and the RiboPrinter Microbial Characterization System (Qualicon Inc., Wilmington, DE) as previously described (17, 18 ).

Pulsed-field gel electrophoresis (PFGE) analysis was performed by using the restriction enzyme XbaI according to the method of Powell et al. (11).

The Fra a 1e gene was cloned into the expression vector pQE70 (Qiagen) by using the restriction sites SphI and BamHI.

Let us now define a general matrix (mathbf {M}) and define the following set of equations: begin{aligned} MA = Aquadquad MB = Bquadquad MSigma M' = Sigma end{aligned} (11)The model is fully identified if the only solution to this system obtained by using the restrictions on A, (mathbf {B}) and (varvec {Psi }) is the identity matrix ((mathbf {M = I})).

The clonal relatedness of the isolates was confirmed by ribotyping using the restriction enzyme EcoRI (Fig. 1), which has a high discriminatory power for K. pneumoniae [23], [24].

(2) Preliminary genetic variability was evaluated by RFLP using the restriction enzymes employed in the previous publication [ 22].

The SULT1A1, Arg213His (R213H) was analyzed by PCR/RLFP using the restriction enzyme Bsp143II to distinguish the G and A alleles according to Engelke et al [ 29].

The 3 bp deletion (intron 6) of GSTM3 was analyzed by PCR/RFLP using the restriction enzyme MnlI according to Inskip et al [ 25].