The activation of NF-κB was monitored by using the Ready-To-Glow Secreted NF-κB Luciferase Reporter Assay (Clontech) as per the manufacturer's instructions.
Viral RNA was amplified into double-stranded DNA by RT-PCR by using the Ready-To-Go RT-PCR Beads (Amersham Biosciences, Piscataway, NJ).
The activation of NF-κB was monitored after 6 hours of stimulation by using the Ready-To-Glow Secreted NF-κB Luciferase Reporter Assay (Clontech) as per the manufacturer's instructions.
Each of these concepts is documented by using the RoundUp Ready® soy GTS40-3-2 GTS40-3-2 GTS40-3-2
Influenza B was detected by using the RealTime ready Influenza B Detection Set (Roche Diagnostics).
DNA sequencing was conducted directly on the purified PCR products by using the Prism Ready Reaction Dye Deoxy Terminator Cycle sequencing kit on an ABI Prism 377 DNA sequencer (Applied Biosystems, Foster City, CA, USA).
Influenza A H1N1 virus in the 124 patients was detected in nasopharyngeal swabs by using the Real-Time ready Influenza A (H1N1) Detection Set (Roche Diagnostics GmbH, Mannheim, Germany) according to manufacturer's protocol.
Expression changes for several genes were confirmed by RT PCR analyses using the Ready-To-Go RT PCR Beads kit (GE Healthcare) with 50 ng of total RNA in 30 cycles.
Sections were then stained by the immunoperoxidase method using the ready-to-use Vectastain Quick kit (Vector Labs, Burlingame, CA).
Sperm protein 17 full-length cDNA (approximately 500 base pairs (bp)) and a control cDNA, β-actin fragment (615 bp), templates were labelled by random priming with [P]dCTP using the Ready-to-Go DNA labelling kit (Amersham Pharmacia Biotech Inc., Pharmacia, Piscataway, NJ, USA).
HA amplicons were sequenced by using the BigDye Terminator Cycle-Sequencing Ready Reaction (Applied Biosystems, Foster City, CA, USA) and ABI Prism 310 DNA sequencer (Applied Biosystems).
