Sentence examples for by using the quantification from inspiring English sources
Further discrimination between true and false positives was enabled by using the quantification cycle (Cq) value of a single mutant template as a cut-off point, thus enabling robust distinction between true and false positives.
Quantification of transcript (mRNA expression) levels was carried out by using the comparative quantification cycle (Cq) method (ABI Prism Sequence Detection System, User Bulletin No. 2 (Applied Biosystems) [ 85].
By using the color quantification q stated above, we define q 1 and q 2 corresponding to the J and K color components.
Fold changes were calculated by using the comparative quantification application of the RotorGene 6000 software.
To quantify the abundance of anammox 16S rRNA in the bacterial populations of both reactors, we performed real-time PCR assays by using the relative quantification method and the digestate as calibrator).
The degree of methylation at each CpG site was calculated as a percentage by using the allele quantification functionality of the PSQHS 96 software and is expressed as a percentage [methylation = peak height methylated/ peak height methylated – peak height nonmethylated) × 100].
The automated quantification of joint effusion from all 25 OA patients gave a mean of 11.7 ± 7.8 ml, whereas 13.5 ± 8.8 ml was obtained by using the manual quantification.
The intensity of labeling in the synovial membrane or a representative portion of cartilage in each image was analyzed by using the IHC quantification technique validated by Pham et al. [ 28].
The NAD+ and NADH levels were measured from whole cell extracts of WT mefs and AMPK α1/α2 double knockout mefs synchronized by with forskolin, by using the Biovision NAD/NADH Quantification kit according to the manufacturer's instruction.
Relative quantification was carried out by using the LightCycler® Relative quantification Software (version 1.0).
Data were collected by using the OneStep Software and relative quantification was performed by using comparative method after determining the Ct (threshold cycle) values for the reference (HPRT1) and the target gene (CaSR) in each sample sets, according to the 2-ΔΔCt method as described by the manufacturer.
