Sentence examples for by using the purified from inspiring English sources

In order to detect Y. pestis, a sandwich immunoassay was developed by using the purified antibody against antigen FI immobilized on polystyrene probes as the capture antibody and the monoclonal antibody-Cy5 conjugate as the detector.

The biochemical properties of BGL were investigated by using the purified recombinant BGL.

The biochemical properties were investigated by using the purified recombinant enzyme.

These bioinformatics data show for the first time that both FXN-2 and FXN-3 may have a biological function and can be considered a starting point for future studies aimed at confirming the function of both FXN-2 and FXN-3 isoforms by using the purified protein.

Another advantage of using the RGEN-RNP system is that designed sgRNAs or crRNAs can be validated for their efficiency for inducing mutations to select the best ones by using the purified Cas9 protein in an in vitro cleavage assay before the actual transfection/injection into cells or embryos is embarked (Sung et al. 2014).

Next, we assessed the reaction rate at lower concentrations by determining the rate of product formation by HPLC, using the purified mixture of authentic 20 a and b as a concentration standard.

Total HPV antibodies were measured by ELISA using the purified HPV type-specific recombinant VLPs.

SsTPx was localized in mite tissue by immunolocalisation using the purified recombinant protein.

The specificity of the assay was confirmed by competitive ELISA using the purified recombinant SAP protein as an inhibiting antigen (Fig. 8B).

We therefore evaluated AHL amide hydrolysis by AtFAAH in vitro using the purified enzyme.

An EGS clone was transcribed by T7 RNA polymerase using the purified EGS-clone IVTT as a template (Fig. 8A, C).