Template (1 μl) was amplified by real-time PCR by using the primers listed in the Table 2 (Integrated DNA Technologies).
One hundred eighty rice cultivars were genotyped at the qSW5 locus by using the primers, and they were divided into 3 types: Kasalath-type, Indica II-type, and Nipponbare-type (Song et al., 2011).
By using the primers designed for qRT-PCR of six OsRUSs (Additional file 6: Table S4), the expression profiles of six OsRUSs at different development stages were generated by qRT-PCR (Fig. 4).
Full EVM025 cDNA was amplified by using the primers: 5'-ACGGGATCCATGGACAATAGTATTTTGTCG-3 (forward) and 5'-TCGGGATCCTCATATCATGTATTTGAGAGTC-3 (reverse).
Control regions of mitochondrial genomes were determined by using the primers developed by Mizi et al. [36].
The two PCR products were fused in frame by using the primers PbF and PfR and resulted in PbfMSP1.
funestus actin gene was amplified as a control for cDNA integrity by using the primers Actin-f and Actin-r (Table S4).
dsRNA was prepared as described [34] from templates prepared by using the primers listed in Table S2 to amplify N2 genomic DNA.
The complete gene for Stx2 was amplified by PCR from total DNA from E.coli O157 H7 C600 (933W) by using the primers previously reported [26].
Transfer constructs for the insertion of a genomic duplication into the pBeloBAC11 sequences within ORF22, ORF50, or ORF54 were generated by using the primers E and F, G and H or I and J.
The PCR products were extracted from agarose gels with a Gel purification kit (Fermentas) and subjected to automated direct DNA sequence analyzes (CRC-DNA Sequencing Facility, Univ. of Chicago, USA) by using the primers described above.
