Primer sets for MDV serotype 1, 2 and 3 were designed by using the Primer Premier 5 software from Premier Biosoft.
Stored sera from all HIV-infected patients enrolled in the study from 2004 to 2009 were tested by EIA, using the Premier Cryptococcal Antigen enzyme immunoassay (Meridian Biosciences).
The primers for genes tsh, iss, cvaC, iroN, iucC, sitA, ompT, hlyF, and etsA were designed from the pAPEC-1 sequence by using the Primer Premier 5.0 program (Table S1).
The data were entered and analyzed by the researchers using the Premier customized data tool [ 21] and IBM SPSS Statistics 20.
Primers were designed using the Premier program (Primer Premier 5).
CDT testing was performed by using the same ELISA (Premier Toxins A&B; Meridian Bioscience, Inc., Cincinnati, OH, USA) throughout the entire period of investigation.
For this retrospective cohort study we used the Premier Perspective database 10 (Premier, Charlotte, NC) containing information on surgical hospital discharges from January 2006 to October 2012.
Additional stool samples were collected serially from day-care attendees, food handlers, and health-care workers whose first stool culture yielded E. coli O157 H7; subsequent stool samples were tested for Shiga toxin by using only the Premier EHEC assay (Meridian Diagnostics Inc., Cincinnati, OH) (8 ).
Gene-specific primers were designed according to the gene sequences using the Primer Premier 5.0 (Premier Biosoft International, Palo Alto, CA).
PCR primers were designed using the Primer Premier v5.0 software (Premier Biosoft, USA).
