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The data were quantified by using the Pathways v.2.01 software (Research Genetics).
Therefore, by using the pathways identified by the path ranking method, we are upscaling both 3M and HME3M to analysis of global metabolic networks.
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Recently, Nair [37] introduced the pathway fractional integral operator by using the pathway idea of Mathai [6], developed further by Mathai and Haubold [38] and defined as follows (cf. [39]).
By using the Pathway GENESWAPPER (see Methods section) and the reference rice seed development network, a gene homology-based projection of Arabidopsis seed development network was developed (http://www.wikipathways.org/index.php/Pathway:WP2279) which was then analyzed for gene loss and gain for inter- and intra-specific comparison (Figure 2).
This is easily possible by using the Pathway Tools comparative module.
In the ConsensusPathDB, we checked the functional annotation of the gene list by using the pathway-based set options.
KAAS annotated a total of 746 UTs with KO terms, and these were further classified into 40 pathways containing 382 UTs by using the pathway identification tool in KOBAS.
We evaluated clinical utility, by using the pathway score as a variable for the warfarin pharmacogenetic equation in the African American cohort (the only cohort with all the pharmacogenetic equation variables available) [ 2].
Reconstruction of the draft metabolic network in P. fluorescens SBW25 was based on the recently published annotated genome of the organism [ 21], and was done in a semi-automated fashion by using the Pathway Tools software suite [ 39], including the HoleFiller algorithm for closing gaps in the metabolic network.
Network analysis was also carried out on the differentially expressed genes by using the Ingenuity Pathways Analysis software (Ingenuity® Systems, Redwood City, CA) [ 26].
We computed these two specificity measures by using the biochemical pathways of E. coli obtained from the KEGG database [ 39] (left panel of Figure 8).
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