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Bound soluble antibody fragments were detected by using the murine mAb 9E10 which recognizes the c-terminal c-myc tag.
The therapeutic potency of the fusion proteins was tested in two consecutive sets of experiments by using the murine AIA arthritis model in C57BL/6 mice.
By using the murine Gnas imprinted domain, he demonstrated that truncating the most upstream Nesp gene, which is transcribed through the whole locus in oocytes, including across the two germline differentially mehtylated regions (DMRs), disrupts methylation acquisition at the DMRs.
We applied the robust Gene Identification Signature PairediTag ditechnologylogy (GIS-PET) to reveal the global patoway aberevealns in melanoma by using the murine melanoma cell line B16F1 as a model system.
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CD4 expression was analyzed by FACS using the murine anti-CD4 antibody SK3-phycoerythrin (Becton Dickinson).
COX-2 overexpression was determined by immunohistochemistry using the murine monoclonal antibody COX-2 (Cayman, USA).
More recent studies by others using the murine model, and BCCA occlusion for 22 min resulted in damage in the hippocampal area as well as the striatal area (Yoshioka et al., 2011b).
The present studies utilized several methods in demonstrating the regulation of StAR protein expression by hCG, using the murine Leydig tumor cell line (mLTC-1) as an experimental model (31).
The effect increased with increasing concentration of antibody and surface CEA, and was lost by using the parent murine IgG1 PR1A3.
In the past years, our group examined the pathogenesis of MM by using the 5TMM murine myeloma models that were valuable in identification of biological processes and new therapeutic targets.
Total RNA was extracted using the RNeasy® Mini Kit protocol (Qiagen, Basel, Switzerland), treated by Deoxyribonuclease I (DNase I) (Invitrogen, Carlsbad, CA, USA), and reverse transcribed by using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT, Invitrogen, Carlsbad, CA, USA).
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