In addition, culture for mycobacteria and direct DST were also performed by using the microscopic-observation drug-susceptibility (MODS) assay (TB MODS Kit, Hardy Diagnostics, Santa Maria, CA, USA) in accordance with published procedures.
Selection and isolation of the candidate cells and/or microscopic areas to be captured was performed under direct microscopic visualisation, by using the Pix Cell II Laser Capture Microscope (Arcturus Engineering, Mountain View, CA, USA).
Varying the optical power P applied to the particle, we measured the trapping force FL and derived the curve of FL(P) versus P. By using the relation curve, we determined the microscopic trapping force FL directly for a given laser power P.
Cells were washed twice with KRB solution and the red fluorescent intensity from podocytes was measured by using the Nipkow spinning disk confocal microscopic system with wavelengths of 514 and 560 nm for excitation and emission, respectively.
Forty-eight hours after transfection, cells on coverslips were excited at 440 nm by using the Nipkow spinning disk confocal microscopic system, and the D1ER fluorescence intensity ratio, which reflects the [Ca2+]ER, derived from measuring two emission wavelengths (F540/F490), was determined using MetaFluor 6.1 software.
For in vivo staining, tumours were cut in 6 -μm sections using a cryostat microtome, mounted on gelatinised slides, processed and analysed by determining the mean pixels intensities of random microscopic fields by using the ImageJ software (NIH).
This was confirmed at a microscopic level and by using the vital dye propidium iodide (which is only taken up by dying cells as their membranes break down) and DAPI which stains all nuclei both in living and dying cells.
Macroscopic constitutive stability measures are introduced corresponding to the positivity of the homogenized moduli tensors relative to a class of conjugate stress strain pairs, and their relations with microscopic stability are investigated by using the proposed theoretical framework.
The microstructural attributes such as state of intercalation/exfoliation/crystalline organization and fractured surface topography of the nanocomposites by using electron microscopic and X-ray diffraction techniques.
The initial dissolution characteristics of an organic soluble benzophenone-containing polyimide (BCPI) film were studied by using a microscopic method and laser interferometry, which could inspect the initial dissolution process at larger and smaller experimental times, respectively.
