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Pathogenic bacteria in blood samples from 21 diseased aquacultural samples were analyzed in a blinded fashion by using the microarray, conventional culture, and 16S rRNA gene sequencing methods; the results of the microarray assay agreed well with those of the conventional culture and 16S rRNA gene sequencing methods.
Transcript levels for CCR7 were the lowest ones among those for the 10 selected genes by using RT-qPCR, and very low signal intensities were measured for CCR7 by using the microarray technology; this indicates that differences in gene expression are more difficult to quantify by using microarray technology for low transcript levels.
Fluorescence intensities were recorded, and data processing was performed by using the Microarray Image Analysis software (Phadia).
By using the microarray technology, which has developed rapidly in last ten years, various gene signatures are developed for various complex diseases, especially the cancer.
Because these functionally related genes might be activated simultaneously upon induction, we analyzed the gene expression changes of gene clusters by using the microarray data.
Transcriptomic analysis of the liver compensatory growth in rodents has been performed by using the microarray [ 24– 27], but gene expression profiles of liver compensatory growth in zebrafish remain to be characterized.
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The sensitivity is defined as the number of sites in which both sequencing and microarrays detected a deviation from the reference sequence divided by the number of sites where a variant was detected by using the microarrays.
These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.
Here we broadly applied the methodology to mapping phenotypes in Nasonia by using the CGH microarray.
The genotypic array analysis was extended by using the barley microarray reference dataset [ 25].
Gene expression values can be imported by using the Cytoscape microarray import feature, for instance.
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