Sentence examples for by using the mega from inspiring English sources

Phylogenic analysis of the different loci was performed by using the MEGA software (version 4.1) [10] after multiple alignments of sequences by CLUSTAL W (1.83) [1.83

The sequences of the isolates were aligned with the reference strains by using the MEGA program v4.0 (Sudhir Kumar, Arizona State University, Arizona, USA) [40]; the resulting reference strain sequences were found to be GenBank sequences under the accession numbers AY184219, AY184220 and AY184221 for Sabin 1, Sabin 2 and Sabin 3, respectively.

Biotin-labelled cRNA was synthesised from cDNA by using the MEGA script In Vitro Transcription Kit (Ambion, Austin, TX, USA).

Multiple sequence alignments were performed by using the MEGA software version 5.2 and the Clustal W program to determine nucleotide and amino acid sequence similarities.

The identity of the sequences, the number of transitions and transversions and the amino acid substitutions were calculated by using the MEGA 4 program [ 51].

The Neighbor-Joining algorithm and the Kimura 2-Parameter model trees, with 1000 bootstrapped replicates, were built by using the MEGA package, version 5.2 [ 39].

A56R-PCR amplicons from 2 exanthematic lesions and 2 milk samples were sequenced in both orientations by using the Mega-BACE-sequencer (GE Healthcare, Little Chalfont, UK).

Molecular data were statistically processed by using the program MEGA (14 ).

Sequence comparisons were carried out using the Basic Local Alignment Search Tool (BLAST) and multiple alignments were performed by using the CLUSTALW (Mega 5.2, Beta version) program [ 39].

All the nucleotide sequences and their alignments have also been listed as supplementary materials (see Additional files 1 2 and 3).The neighbour-joining (NJ) tree was constructed by using the software MEGA 4.02 with the substitute model of Maximum Composite Likelihood [ 42].

Phylogenetic trees were conducted by the neighbor-joining method using the MEGA 3.1 program.