The macromonomer branch length was controlled by using the living anionic polymerization technique.
New well-defined block polymers and star-branched polymers with reactive poly(1,4-divinylbenzene) segment(s) could be successfully synthesized by using the living anionic poly(1,4-divinylbenzene).
Percent cell death was measured by using the Live-Dead Assay according to the manufacturer's instructions (Molecular Probes, Eugene, OR) with a Gemini XS fluorimeter (Molecular Devices, Sunnyvale, CA).
Captured images were then quantified by using the Living Image software (Xenogen Corp ,Almeda, CA) and the IGOR software (WaveMetrics Corp ,Lake Oswego, OR).
Halper was a painter for most of her adult life and was always intrigued by using the private lives of her children and their social circles for inspiration.
For bioluminescence quantification, regions of interest (ROI) were drawn by using the software Living Imaging 3.0 and average radiance (p/s/cm2/sr) was determined.
The wells were then rinsed with fresh media to remove floating cells, and the wound healing process was monitored continuously by using the IncuCyte live-cell imaging system (Essen BioScience, Ann Arbor, Michigan).
These are streams that have been found by others and can be watched using the Live station player.
The activity of caspase-3 was measured by using the Image-iT LIVE Green Caspase-3/7 Detection Kit (Invitrogen).
Similarly, we calculated birth weight-and gestational age-specific early neonatal, neonatal, post-neonatal and infant mortality rates (as per convention) by using the number of live births at each birth weight or gestational age as the denominator.
A first index for accounting the inflation in Puerto Rico was born by using the cost of living for working families in 1940.
