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High-speed lithium flow was demonstrated in free surface condition by using the lithium loop facility at Osaka University.
The direction of phaC insertion was confirmed by colony PCR with primer 3 and 4. Yeast transformation was performed by using the lithium acetate/single-stranded carrier DNA/ polyethylene glycol method (Gietz and Woods 2002).
Yeast transformations were achieved by using the lithium acetate/single-stranded DNA/polyethylene glycol method as previously described (Gietz and Schiestl, 2007).
After a second PCR for elongation of the fragment, S. cerevisiae EBY100 (Invitrogen) was transformed with the resulting PCR fragment (containing the mutagenized AB- and EF-loop sequences) and the linearized pYD1-Fc vector (missing part of the CH3 domain: AB- and EF-loops and the fragment in between) by using the lithium acetate method [ 12].
Similar(56)
Measurement of extravascular lung water (EVLW) by using the lithium-thermal (Li-thermal) and single-thermal indicator dilution methods was compared with the indocyanine green-thermal (ICG-thermal) method in humans.
Novel small-mesopores-enriched porous carbon microspheres have been synthesized from carbonaceous polysaccharide microspheres, by using the associated lithium acetate treating and heat treating strategies.
The amplified PCR product was used for yeast modification by homologous recombination following yeast transformation using the lithium acetate procedure.
These experiments have been performed by using the following salts: lithium chloride, sodium chloride, potassium chloride and cesium chloride.
This clinical report presents a protocol for the fabrication and delivery of an endocrown by using the biogeneric design mode with lithium silicate-based ceramic adjusted before its complete sintering.
In this paper we will present the analysis of thin-film BAW resonator designed in 2D using eigenfrequency, by using the Aluminium Nitrid (AIN) and Lithium Niobate (LiNbO3) materials and try to achieve a high quality ( Q) factor compared with bibliography.
The aim of this study was to compare measurements of ITBV and EVLW volume made by using the indocyanine green-thermal (ICG-thermal), lithium-thermal (Li-thermal), and single-thermal indicator dilution techniques in patients after elective cardiac surgery with cardiopulmonary bypass.
More suggestions(15)
by using the content
by using the cell
by using the primary
by using the cells
by adding the lithium
by coupling the lithium
by protecting the lithium
by adjusting the lithium
by controlling the lithium
by permitting the lithium
by combining the lithium
by shortening the lithium
by varying the lithium
by immersing the lithium
by recovering the lithium
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