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The samples were analyzed by using the Liaison kits (Byk-Sangtec, Dietzenbach, Germany).
As for plasma 25(OH D, it was measured through chemiluminescence assay by using the Liaison 25-hydroxyvitamin D Total assay (DiaSorin Inc., Stillwater, MN, USA).
We determined 25(OH D levels by using the Liaison 25OH vitamin D TOTAL (Diasorin Inc., Stillwater, MN, USA), a chemiluminescence immunoassay with an interassay coefficient of variation of 7.0% at 18 ng/ml and 6.3% at 37 ng/ml.
Samples for procalcitonin (PCT) were analyzed in a subgroup of 48 patients by using the LIAISON BRAHMS 2-site immunoluminometric assay (Liaison Brahms PCT; Brahms Diagnostics, Berlin, Germany), run on the Diasorin Liaison instrument.
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Circulating 25(OH D was measured by Heartland Assays, Inc. (Ames, IA, USA) by using a direct competitive chemiluminescence immunoassay by using the DiaSorin LIAISON platform (DiaSorin, Inc., Stillwater, MN, USA) [ 23].
As reported elsewhere (18), all serum or plasma samples were assayed for 25(OH D at Heartland Assays, Inc. (Ames, Iowa) by a direct, competitive chemiluminescence immunoassay by using the DiaSorin LIAISON 25 OH Vitamin D TOTAL Assay (19, 20).
Specific insulin or C-peptide measurements were made using the Liaison C-peptide and insulin tests on a Liaison analyzer (DiaSorin, Saluggia, Italy).
Insulin and C-peptide were measured using the Liaison C-peptide and insulin luminescence immunoassay on the Liaison analyzer (DiaSorin, Saluggia, Italy).
Samples had been preclassified using the Liaison® CMV IgG and CMV IgM assays (DiaSorin, Saluggia, Italy).
Samples were assayed using the LIAISON 25-OH 25-hydroxyvitamin D TOTAL (DiaSorin, Italy).
Total 25-hydroxyvitamin D (25-OHD) was measured using the LIAISON analyzer (DiaSorin Inc., Stillwater, MN).
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