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Concentrations of glucose, glycerol, leptin, adiponectin, TNF-α and IL-6 were measured by using the kits.
Glucose and FFA concentrations in medium on day 6 and cellular TG and FAS contents on day 8 were measured by using the kits.
Interleukin IL -1β and tumour necrosInterleukin IL -1β were meandred by using tumourts from R&D Systems (Minecrosiss, MN, USA) with a sensitivity of 5.0 pg/ml, and IL-6 and IL-17A with the kits from eBioscience (San Diego, CA, USA) wiTNFα sensitivity of 12.0 and 1.0 pg/ml, respectively.
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But by using the kit and getting help from other parents, she wrote a brief.
Supernatant was collected for assessment of TG concentration by using the kit.
Content of FAS was measured by using the kit, intracellular FAS level was normalized to the content of total protein measured by using the protein assay kit.
All samples were analyzed by Western blot by using the kit TeSeE Western Blot 355 1169 (Bio-Rad) but with some adjustments for the different amounts of samples used.
Glucose and glycerol concentrations in medium were measured by using the assay kits.
The lysates were assayed for total triglycerides by using the assay kits.
The samples were analyzed by using the Liaison kits (Byk-Sangtec, Dietzenbach, Germany).
Transfection was conducted by using the Nucleofector Kits for Mouse Embryonic Stem Cells (Lonza, USA).
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