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But by using the kit and getting help from other parents, she wrote a brief.
Supernatant was collected for assessment of TG concentration by using the kit.
Content of FAS was measured by using the kit, intracellular FAS level was normalized to the content of total protein measured by using the protein assay kit.
All samples were analyzed by Western blot by using the kit TeSeE Western Blot 355 1169 (Bio-Rad) but with some adjustments for the different amounts of samples used.
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Concentrations of glucose, glycerol, leptin, adiponectin, TNF-α and IL-6 were measured by using the kits.
Glucose and FFA concentrations in medium on day 6 and cellular TG and FAS contents on day 8 were measured by using the kits.
Total RNA was extracted by using the RNeasy Kit (Qiagen, Venlo, The Netherlands) according to the manufacturer's instructions.
Spoligotyping was carried out by using the commercial kit from Isogen Bioscience BV (Maarssen, The Netherlands) [ 10].
Nested PCR was performed by using the Expend High Fidelity PCR kit (Roche).
RNA was extracted by using the YRB50 kit (RBC bioscience) according to the manufacturer's protocol.
Genomic DNA was extracted from cord-blood leukocytes by using the Nucleon kit (Amersham).
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CEO of Professional Science Editing for Scientists @ prosciediting.com