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Western blots were done as previously described by using the indicated primary antibodies and an alkaline phosphatase-conjugated anti-IgG secondary antibody (Sigma) [ 23].
A logistic regression model was performed by using the indicated predictors including fever duration (days) or 1st IVIG used time (days after the first date with fever).
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The various resulting extracts were centrifuged at 10000 × g for 30 min at 4°C and TSPs were separated on 12 to 15% SDS polyacrylamide gels and the proteins were detected by staining or by immunoblot using the indicated antibodies.
Samples were separated by SDS-PAGE, followed by immunoblotting using the indicated antibodies.
The various resulting extracts were centrifuged at 10000 × g for 30 min at 4°C, and proteins were separated on SDS polyacrylamide gels and detected by Coomassie blue staining or by immunoblot using the indicated antibodies.
Chromatin immunoprecipitation from the indicated cell lines and using anti-Fra1 or control (no antibody) was quantified by qPCR using the indicated primers.
The proteins were detected by immunoblotting using the indicated antibodies.
The lysates and immunoprecipitates (IP) were analyzed by immunoblot using the indicated antibodies.
The resulting samples and lysates were analyzed by immunoblotting using the indicated antibodies.
The endogenous ubiquitin and SUMO2/3 conjugations of global proteins were determined by IB using the indicated antibodies.
Samples were analyzed by immunoblotting using the indicated antibodies.
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