Sentence examples for by using the extinction from inspiring English sources

Approximate formulas for the absorption cross-section are derived by using the extinction theorem.

APX activity was calculated by using the extinction coefficient 2.8 mM−1 cm−1 and expressed as µmol decomposition of ascorbate min−1 mg−1 protein.

Substrate concentration was determined by using the extinction coefficients 5438 L/(mol·cm) at 336 nm (Edans) or 15,100 L/(mol·cm) at 472 nm (Dabcyl).

High concentrations of 0.3 1.0 N NaOH had to be applied to denature mRuby to determine the chromophore concentration by using the extinction coefficient of denatured GFP at pH 13 [37].

The protein concentration was estimated by BCA assay (Bio-Rad, Hercules, CA, USA), as well as spectrophotometrically at 280 nm by using the extinction coefficients calculated from the amino acid sequences of the constructs.

The reaction mixture contained 33 mM H2O2 in 50 mM phosphate buffer pH 7.0 and the activity of CAT was calculated by using the extinction coefficient of 40 mM−1 cm−1 for H2O2.

A rough estimate of the cytochrome c and cytochrome a concentrations in sample cuvettes containing solubilized membranes was achieved by using the difference extinction coefficients Δε552-538 = 17.3 mM−1×cm−1 [32] and Δε604-630 = 25.2 mM−1×cm−1 [86], respectively.

The concentrations of the recombinant proteins were measured by UV at 280 nm in 20 mM sodium phosphate (pH 6.5) and 6 M guanidine HCl by using the theoretical extinction coefficient calculated with EXPASY software (http://us.expasy.org/tools/protparam.html).html

The concentrations of the recombinant proteins were measured by UV absorbance at 280 nm in 20 mM sodium phosphate, pH 6.5 and 6 M guanidine HCl by using the theoretical extinction coefficients calculated with EXPASY software (http://us.expasy.org/tools/protparam.html).html

The concentrations of the recombinant proteins were measured by UV radiation at 280 nm in 20 mM sodium phosphate, pH 6.5 and 6 M guanidine HCl by using the theoretical extinction coefficients calculated with EXPASY software (http://us.expasy.org/tools/protparam.html).html

Protease concentrations were determined by using the appropriate extinction coefficient (E1%/280 = 17.65 thermolysin, E1%/280 = 14.7 pepsin).