By using the expand button the data from the other reports are also shown.
RNA was reverse transcribed by using Superscript II (Invitrogen), and the cDNA was amplified by using the expand high fidelity PCR system (Roche, Indianapolis, IN, USA) according to manufacturer's instructions.
The RT reaction was performed at 42°C for 60 min. The cDNA was amplified by using the Expand Long Template PCR System (Roche Diagnostics, Laval, QB, Canada), following the protocol provided.
FJ983127) was obtained after LongRange PCR by using the Expand Long Range dNTPack (Roche, Mannheim, Germany) according to the manufacturer's directions and the following 2 primers: ABS F1-B (5′-AAAAgAggCAAAgCAAgACAgTgg-3′) and ABS R2-B (5′-ggCgggCAACAAAgACCTCA-3′).
The mixture was frozen at −80°C for 10 min, followed by incubation at 60°C for 1 h, and proteinase K was inactivated at 95°C for 15 min. Using a modification of a PCR protocol (43), animals were genotyped for the expanded CGG repeat by using the Expand Long Template PCR System (Roche Diagnostics Corporation, Indianapolis, IN, USA).
Functional disability status was also assessed by the treating physician using the Expanded Disability Status Scale (EDSS, Table 1) 26 and/or the Karnofsky Performance Scale (KPS, Table 2).
For well preserved DNA, the mitochondrial genome was amplified in two overlapping pieces around 11 and 6 kb in length, by long range PCR using the expand dNTPack (Roche) according to the manufacturer's instructions.
Here the program included suites from "Figaro" and "Don Giovanni," played in arrangements by Mr. Bernardini that used the expanded palette of the "Gran Partita".
Their rheological behaviors, especially the sol gel transition were investigated by using the advanced rheological expanded system on the basis of Winter and Chambon theory.
