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Reverse transcriptase (RT) was performed by using the Enhanced Avian RT First Strand Synthesis Kit (Sigma) with Anchored oligo dT) (Thermo Scientific and PCRR nucleotide mix (Fisher) to generate cDNA by using 2 μg of RNA.
Results indicate that by using the enhanced method, computation times can be substantially reduced, especially for the larger problems.
The results demonstrate that the two challenging issues, surface finish and geometrical accuracy, could be improved by using the enhanced technologies of E-DSIF.
To evaluate the proposed method, one data set is processed separately by using the enhanced analytic signal method and the multivariate EMD (MEMD) algorithm, and the results from these two methods are compared with that of the proposed method.
In this subsection, we will solve Eq. (1) by using the enhanced modified simple equation method.
Using subcritical water to mobilise heavy oils requires a balance to be found between (1) dissolving the heavy oil component within this novel medium (determined by dielectric constant ε) and (2) cracking it simultaneously by using the enhanced [H+] and [OH−], which are present.
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The nuclear localization of p65 was automatically quantified by using the Enhanced-Translocation module of MetaXpress (Molecular devices, Sunnyvale, USA).
Secondary antibodies conjugated to HRP (AbCam, Cambridge, UK) were used, and the signals were detected by chemiluminescence using the Enhanced ECL kit (GE, Amersham, UK).
In current work the direct growth of graphene by using the Plasma Enhanced Chemical Vapor Deposition PECVDD) technique was demonstrated to make fabrication more practical on a large scale.
The LNA NF can be further reduced by using the second generation enhanced SiGe C HBT BiCMOS process.
Finally, transgene expression by the three DNA forms were investigated by using the reporter gene Enhanced Green Fluorescent Protein (EGFP).
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