Sentence examples for by using the dna from inspiring English sources
It is noteworthy that more than 80percentt of the matches New York City now achieves by using the DNA database in sexual assault cases are linked to offenses that are not sex related, many of them fairly mundane.
These results suggest that two nosocomial pathogens, P. aeruginosa and A. baumannii, can be efficiently diagnosed by using the DNA microarray developed in this study.
By using the DNA sequences, the VH and VL gene families of the scFvs were designated based on Werner Müller's database (DNAPLOT software).
Patient demography is shown in Table 2. Total genomic DNA was isolated from the biopsies by using the DNA mini kit (Qiagen, Valencia, CA, USA) with glass beat beater method as previously described [16].
‡ Sequence divergence analyzed by using the DNA distance matrix in BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html).html
*Pairwise nucleotide divergence was calculated by using the DNA distance matrix in BioEdit (www.mbio.ncsu.edu/BioEdit/bioedit.html).html
It was found by using the DNA-binding domain (DBD) of Estrogen Receptor α (ERα) as a hybridization probe to screen recombinant DNA libraries [1].
RNA samples for semi-quantitative PCR were treated with DNase by using the DNA-Free Turbo kit (Ambion); reverse transcription reactions were then performed using random hexamers and Superscript III (Life Technologies) by following manufacturer's directions.
Genomic DNA was removed from RNA by using the DNA-free kit (Ambion®, Austin, TX) in order to prevent an amplification of the PTEN pseudogene.
The RNeasy RNA Isolation Kit (Qiagen) was used to isolate RNA from each sample, following manufacturer's instructions except the final step where genomic DNA was removed by using the DNA-free Kit (Applied Biosystems) per manufacturer instructions.
For molecular analysis, cultivated bacteria were resuspended in saline and inactivated at 98°C for 20 min. Total DNA was extracted by using the DNA-purification Kit (QIAGEN, Hilden, Germany).
