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In brief, NET-associated MPO in serum or culture supernatant was captured by using the coated 96-well plate of the human MPO ELISA Kit, (Hycult Biotech), after which the NET-associated DNA backbone was detected by using the detection antibody of the Human Cell Death Detection ELISAPLUS (Roche Diagnostics).
In later sections, by using the detection probabilities derived from our theoretical analyses, we present numerical values for, and.
In[2], the optimum node density of a many-to-one linear network is analyzed by using the detection probability of a binary event as the performance metric.
The amounts of PBMC-derived HIV-1 DNandnd RNA were normalized to total cellular inputs, which were quantified in separate real-time PCR by using the detection kits for either beta-actin or ribosomal RNA, respectively (both Applied Biosystems), and expressed either as number of copies per 106 PBMC for prDNA, or as number of copies per µg total RNA for usRNA and msRNA.
Terminal DNA transferase-mediated dUTP biotin nick end labeling (TUNEL) assay was employed to determine in situ apoptotic DNA breaks [ 21, 22] by using the Detection Kit (Roche Applied Sciences, Germany) following manufacturer's instructions.
The first hypothesis was tested in terms of whether blastocoelar GADCs proliferate by using the detection of BrdU, which was applied during the period of active increase in the number of blastocoelar GADCs; however, the obtained result was an insubstantial increase in the proportion of BrdU-incorporated blastocoelar GADCs (Fig. 5A,B).
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All patients were also classified using socio-demographic variables (age, gender, marital and family status), collected by GPs using the detection module.
Immunoreactivity was detected by using the ECL detection system and semi-quantified with gel image analysis software.
Suspended cells such as red blood cells and white blood cells have been successfully detected and distinguished by using the blob detection method [22].
No recombination event was detected within the TLS25 strain by using the Recombinant Detection Program (http://darwin.uvigo.es/rdp/rdp.html).es/rdp/rdp.html
Pi2 pulsations are selected in 2100 0100 magnetic local time by using the automated detection algorithm developed by Nosé et al. (1998).
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