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The real-time value for each sample was averaged and compared by using the CT method.
For this sequence with moving events, the reduction in power was roughly one half by using the CT to gate the processing of blocks.
Levels of mRNA were normalized to β-actin and calculated by using the Ct method.
Finally, the difference electronic density calculations were performed by using the CT program.
This needs to be corrected by using the CT as the anatomical template and subsequently 'trimming' part of the GTVpet which is clearly in an air cavity.
and 60°C for 1 min. Relative expression was determined by using the CT method (Pontius et al., 2003) using Sequence Detector software, version 1.6.3.
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Conformality can be improved by using the CT-based 3D-HDR-BT treatment plan, and we hypothesized that improvements in LCS and DFS with 3D-HDR-BT were due to the conformal dose distributions [ 3, 20].
The relative amounts of the different mRNAs were quantified by using the comparative CT method (2− ΔΔ CT )).
Relative expression levels for each desired gene were normalized against the Ct value of GAPDH and determined by using the delta Ct method.
In general, performance of CT registration might be improved by using the original CT images that were not downsampled to the PET resolution.
The amount of specific mRNA was quantified in unknown samples by using the comparative Ct method: the ΔCt values from each tissue were obtained by subtracting the values for 18S Ct from the Ct of each tested gene.
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