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24 Briefly, the differential expression level of all genes between TGF-β-treated samples and non-treated samples was computed and the top 5% of upregulated genes in TGF-β-treated cells compared with control cells were selected by using the "Comparative Marker Selection" tool from each dataset.
In this paper, 3-dimensional quantitative structure activity (structure affinity and structure efficacy) relationships are investigated for a series of non-steroidal agonists (fexaramine series) by using the comparative molecular field analysis (CoMFA), where molecular docking method (FlexX) is employed to construct molecular superimposition maps.
Expression profile in each specimen was assessed by using the comparative threshold cycle (2-ddCt) method.
Samples were run in triplicate, and relative quantification was performed by using the comparative CT method [14].
Quantitative values were obtained by using the comparative threshold cycle (ΔΔCT) method, as described in Livak et al. [98].
The relative mRNA abundance was determined by using the comparative critical threshold method and is expressed relative to the control diet group [96].
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The transcribed audiotapes were analyzed by theme linkage by using the constant comparative method.
Data from the NRLS, specifically the free-text elements, were analysed thematically by using the constant comparative method [ 14].
Seven hundred nineteen serum samples were tested by using the NiV comparative ELISA, of which 716 (99.6%) were negative and three (0.4%) had an inconclusive test result.
Identification of the exon/intron organization of SWEET genes was performed by aligning cDNAs with their corresponding genomic DNA sequences and were also obtained by using the Plaza comparative database.
The target gene expression levels were determined by relative quantification using the comparative CT method, also known as 2-∆∆CT method (comparative 2 [delta][delta]Ct method).
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