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We used the ρ statistic (mean sequence divergence from the inferred ancestral haplotype of the clade in question) with a mutation rate estimate for the complete mtDNA sequence of one transition in every 3,624 years [ 31], correcting for purifying selection by using the calculator provided with that paper.

The dates of the most recent common ancestor of specific subclusters in the phylogeny were estimated using ρ, the average number of transitions from the ancestral sequence type to all sequences in the cluster, based in the recently updated mutation rate published by [ 36] for the entire molecule (1 mutation in every 3624 years), and by using the calculator provided in the paper.

You can get an estimate of your Social Security income by using the calculators at the Social Security Administration Web site.

All the indexes of the input parameters were calculated by using the raster calculator with the output cell size was 67.08 m.

They are cationic peptides mainly enriched by residues such as lysine hystidine and arginine, with a net charge of +0.9, calculated by using the protein calculator v3.3.

Confidence intervals were calculated by using the online calculator for screening on Open Epi (4 ).

For testing the null hypothesis that the two means of gene expressions between LPS treated seabass and control were equal, a two-tailed T-test was performed by using the web calculator 'Independent groups T-TEST for means calculator' [ 92].

Unpaired t-tests were used to determine the statistical significance by using the online calculator available at the GraphPad Software website (http://www.graphpad.com/quickcalcs/ContMenu.cfm).

We concatenated the ORFs present in all isolates and calculated the Ks values according to the modified version of the Yang-Nielsen algorithm (MYN) by using the KaKs calculator 2.0 (Zhang et al. 2006; Wang et al. 2010) for deeper analysis of the evolutionary history.

Isoelectric points of OPN virtual peptides were determined using the calculator developed by Gauci and coworkers.