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The MIC values for these polymers were determined by using the broth dilution method.
The minimum inhibitory concentration (MIC) of 12 antibiotics was determined for four strains of staphylococci (representing the three major species) under aerobic and anaerobic conditions by using the broth dilution method.
Data were interpreted by using the broth microdilution criteria (7 ).
The minimal inhibitory concentration (MICs) and minimal bactericidal concentration (MBCs) were determined by using the broth macrodilution method.
The minimal inhibitory concentration (MIC) of the extract and its fractions was determined by using the broth microdilution technique [ 15].
All MICs were determined by using the broth microdilution method with Mueller-Hinton II cation adjusted broth (Difco, Franklin Lakes, NJ, USA).
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Cultures of hand samples were done by using the broth-bag technique (1).
MICs of 15 antimicrobial drugs (Table) were determined by using the broth-microdilution method; susceptibility to streptomycin was measured by using the disk-diffusion method as recommended by the Clinical and Laboratory Standards Institute (4 ).
These results suggest that succinic acid can be purified in a cost-effective manner by using the fermentation broth of engineered LPK7 strain, showing the importance of integrating the strain development, fermentation and downstream process for optimizing the whole processes for succinic acid production.
Drug susceptibility was determined by using the reference broth microdilution method described by the Clinical and Laboratory Standards Institute (11 ).
Confirmatory susceptibility testing of isolates was conducted by using the Clinical Laboratory Standards Institute broth dilution method (9 ) (Table).
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