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Finally, by using the background of the Cys-less PrnB transporter, we evaluate the functional importance of amino acids Q219, K245 and F248 of PrnB, which our previous data had suggested to be involved in the mechanism of PrnB-mediated proline uptake.
E-values for the traditional structural alignment are derived by using the background information, i.e., the distribution of alignment scores for random proteins that are structurally dissimilar to the query protein.
The actual numbers in terms of content of predicted long disorder decreased from ∼18% for the native to ∼9% for the final mutant by using the background mutation protocol (Fig. 3C, green).
We demonstrate how to test the statistical significance of the motifs discovered via the graph pruning/mathematical programming approach by using the background frequencies for each base or amino acid and computing the motif scores' probability distribution [ 38].
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Cycle threshold values were calculated for each sample automatically by the QPCR System's software by using the background-based method.
Next, a third-order background model was generated for each species from all promoter regions by using the background-generator program provided with MotifSampler.
He believes LetsTransport can set itself apart by using the combined background of its founding team, which also includes former J.P. Morgan equity strategy analyst Sudarshan Ravi and software engineer Ankit Parasher, "to cater to the varying needs of multiple dissimilar stakeholders and industry verticals".
Surface areas were estimated by either integrating the current and multiplying by time or using the background current at 0.25 V.
Ntirogiannis et al. [21] proposed a background reconstruction method and computed normalized images by using the reconstructed backgrounds.
"We start by assessing the identified problems, using the background knowledge in drug therapy.
(Participant 001) "We start by assessing the identified problems, using the background knowledge in drug therapy.
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