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Hoechst-stained nucleus was counted by using the "Analyze particles" tool in ImageJ software.
To analyze individual laminin aggregates, the numbers and integrated density were determined by using the "Analyze Particles" function.
Quantification was performed by drawing a line that covers the length of each villus and then by using the "Analyze > Set Scale" function of the program to determine the length in pixels.
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An interpolation method was introduced to calculate SIF of each model in a valid range by using the analyzed SIF results in the database.
Receiver operator characteristic (ROC) curves were constructed by using the Analyze-it software (Analyze-it Software Ltd, Leeds, UK), and correlation between ELISA and microarray results was calculated by using the JMP software (SAS Inc., Cary, NC, USA).
The determination of operational stability and inactivation kinetics of the constructed biosensors was performed by using the analyzing system "Olga" (output of sensors to 1 mM ethanol, ejection time 2 min) [ 19].
Protrusion and retraction areas were analyzed by using the ImageJ Analyze Particle plug-in.
By using the theory we analyze separation of polymers by molar mass, functionality and topology.
Size distribution measurements for Au particles were performed on digital images by using the image analyzing software Image-Pro.
Categorical variables were analyzed by using the Fisher exact test; continuous variables were analyzed by using the Wilcoxon rank-sum test (because none were normally distributed).
Continuous variables were analyzed by using the Mann–Whitney U test and discrete variables were analyzed by using the Chi squared test.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com