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Immunohistochemical staining was performed with mouse antihuman ER-α 1D5 by using the alkaline phosphatase antialkaline phosphatase technique.
The initial rate of reaction in the aeration process is controlled by using the alkaline solution (viz., sodium hydroxide (NaOH) and sodium silicate (Na-Si)) which is cooled to a lower temperature.
The effect of different organic solvents on heme solubility was assessed by two different approaches: the first one by measuring the light absorption of the Soret band and the second one by using the alkaline pyridine method [28].
Alkaline phosphatase activity was detected by using the Alkaline Phosphatase Substrate III kit (SK-5300; Vector Laboratories).
In this study, the reduction in pH by washing or neutralization was omitted by using the alkaline character of LTWS to neutralize lactic acid produced by microbial fermentation during a SSF process.
Immunohistochemical detection of natural killer (NK) cells in the rat liver was performed by using the alkaline phosphatase-anti-alkaline phosphatase (APAAP) method as described previously in more detail (Batusic et al. 2005).
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Serum creatinine concentrations were measured at the White Sands Research Center Coulston Foundationn) laboratory (Alamogordo, New Mexico) by using the kinetic alkaline picrate reaction method on a Roche/Hitachi 737 analyzer (Roche Diagnostics, Indianapolis, Indiana).
DNA single-strand breaks produced by tirapazamine were measured using the alkaline comet assay as previously described (Olive, 1995).
Plasma ALP was measured by a kinetic photometric test using the Alkaline Phosphatase FS kit supplied by DiaSys Diagnostic Systems GmbH (Germany) according to the method described by Moss and Henderson [ 8].
It was also found that the quality of the biodiesel produced using the acidic catalyst was better than that produced using the alkaline catalyst by having higher amounts of C16 : 0 and C18 : 3.
Bound antibodies were detected by using the streptavidin-biotin-alkaline phosphatase method and New-Fuchsin as chromogen.
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