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RNA was extracted from specimens by using the acid guanidinium phenol method (19 ).
Total RNA extraction was performed from frozen tumor specimens by using the acid guanidinium isothiocyanate/phenol/chloroform procedure.
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Total RNA was extracted from breast tissue by using the acid-phenol guanidium method, and its quality was determined by agarose gel electrophoresis and ethidium bromide staining.
Total RNA was extracted from breast tumor samples by using the acid-phenol guanidium method.
Total RNA was extracted from breast specimens by using the acid-phenol guanidium method.
Total RNA was extracted from breast specimens by using the acid-phenol guanidium method (Chomczynski and Sacchi, 1987).
Insulin-like growth factor-I was extracted from IGF-binding proteins (IGFBPs) by using the acid-ethanol extraction method.
Total RNA was extracted from frozen tumor samples, MPNSTs cell lines, and fresh adult nerves by using the acid-phenol guanidinium method.
Cells were shown to be tartrate-resistant acid phosphatase (TRAP -positive by sTRAP -positivethe Acid Phosphatase Leukocyte Kit (Sigma-Aldrich, Seelze, Germany) with an adapted protocol.
A dose response linear regression was generated by using the gallic acid standard absorbance and the levels in the samples were expressed as gallic acid equivalents (mg of GAEs/g of extract).
A dose response linear regression was generated by using the gallic acid standard absorbance and the levels in the samples were expressed as gallic acid equivalents (mg of GAEs/mg dw).
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