Digestion of cells was accomplished as described above, followed by total RNA isolation using the Absolutely RNA Nanoprep Kit (Stratagene).
Total RNA was isolated using the Absolutely RNA Miniprep kit (Stratagene) and reversed transcribed by the Moloney murine leukaemia virus reverse transcriptase (Invitrogen Life Technologies) as previously described [ 43].
RNA was extracted by hot phenol lysis (Gasch 2002) and mRNA was twice purified from total RNA using the Absolutely mRNA purification kit (Stratagene).
RNA was isolated using the Absolutely RNA®RT-PCR Miniprep Kit (Stratagene) and RNA quality was measured by a 2100 Bioanalyzer (Agilent).
Total RNA from laser-captured tissue was isolated using the Absolutely RNA Nanoprep kit (Stratagene, Amsterdam, The Netherlands).
Total RNA was isolated using the Absolutely RNA Kit with DNase on-column treatment (Agilent Technologies, La Jolla, CA USA).
Sample pellets were stored overnight at −80°C, followed by RNA preparation using the Absolutely-RNA kit (Stratagene, La Jolla, CA) according to the manufacturer's instructions.
Observe that the series is absolutely convergent by using the ratio test and hence the series in (17) is absolutely convergent.
And (mathbb{N}={0, 1, 2,ldots }), by w we denote the space of all real sequences and θ is the zero vector of E. In [1], by using the approximation numbers and p-absolutely summable sequences of real numbers (ell^{p}) ((0< p
In fact, some experts suggest that blowing your nose can lead to a build up of pressure due to trapped mucus, which causes further damage to your nasal passage.[14] You can avoid this by blowing only when absolutely necessary, and by using the correct blowing method.
