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We conducted a comprehensive survey on the transcription profiling database by using tBLASTn analysis.
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Initial identification of two GATA gene fragments was conducted using tBLASTn analysis of the B. floridae trace archive.
We used TBLASTN analysis to compare predicted proteins of LF82 strain with the complete DNA sequence of the other strains.
The EST database of G. sulphuraria was searched against by using tBLASTn program.
For this purpose the genome sequence of model organisms (see Additional file 9) were also searched by using tBLASTN [ 75].
The N-terminal sequences VEIKXQLSGLWENEQDSLMEISALADDGG and VERKXQLSGLWENEQDSLMEISALADDLEN [ 4] were used to search the deposited collection of chicken cDNAs [ 15] by using TBlastn at the NCBI web site.
The candidate T3SS effectors in the XauB and XauC genomes were identified using tBLASTn [ 45] analysis and Pfam domain [ 111] searches.
The absence of any homologue in a given lineage was systematically verified by hand using tBLASTn searches on the nucleotide sequences of the corresponding complete genomes.
To evaluate the efficacy of our tblastn + Pfam-based screen, we conducted a receiver operating characteristic (ROC) analysis using tblastn data from all species surveyed [ 25].
Similarity analysis using tblastn program indicates that the Glyma05g03490 gene shares suggestive homology (55% identity and 70% similarity) with the rice locus Os06g0127800 gene encoded protein.
However, the orthologous regions of both dog and chicken genome assemblies possess no PRAME homologous genes, as determined by searches of these regions using TBlastn [ 25].
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