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Aiming at fundamental elucidation of the molecular mechanisms involved in N starvation-induced TAG accumulation, the global gene expression changes were identified by using suppression subtractive hybridization (SSH).
The objective of this study was to identify R. microplus genes differentially expressed in male salivary glands in response to infection with A. marginale by using suppression subtractive hybridization (SSH) approach and to characterize the function of those genes by RNAi.
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We further identified a total of 74 chrysanthemum unique transcripts induced by AtDREB1A under drought stress using suppression subtractive hybridization [ 10].
All serum samples were negative by microneutralization assay which uses suppression of virus antigen expression as endpoint, using the recommended cut off values of ≥1∶80, except for two samples with titers of 1∶80 and 1∶350.
Second, to enrich for fungal genes expressed during colonization and propagation within the plant cells, as well as plant genes induced by the invading pathogen, we used suppression subtractive hybridization technology [ 22]. cDNAs synthesized with RNA from uninfected rice leaves were used as the driver, while those from heavily infected rice leaves were used as the tester.
The estimated noise PSD is used by the postfilter to suppress the noise and residual echoes by using a suppression filter that is presented in the next section.
By using the suppression subtractive hybridization (SSH), a cDNA library of rice leaves induced by sheath blight fungus was constructed, and 63 high quality EST sequences were generated.
Okita et al. [10] further improved the episomal vector by using p53 suppression and non-transforming L-Myc instead of c-Myc, resulting in markedly enhanced iPS generation (1 × 10−5 3 × 10−4).
We previously screened and cloned lncRNA urothelial cancer-associated 1 (UCA1) by using subtractive suppression hybridization technique from two human bladder transitional cell carcinoma cell lines, BLS-211 and BLZ-211, which were derived from the same patient's sample but with different biological characteristics.
Bone marrow contrast can be accentuated by using fat-suppression (fs) sequences.
Suppression subtractive hybridization (SSH) [ 5] overcomes this by using an additional suppression PCR step that provides 10-100 fold enrichment of differentially expressed mRNAs, irrespective of their relative abundance.
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