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Effect of roughness on the accuracy of the measurement was tested by using standard targets of known roughness values.
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HPLC-MS technique is an important method used for identifying complex mixtures, especially the phenolics or its fraction found in the plant, either by using standard compounds (target identification) or by comparing mass spectrum obtained with literatures (tentative identification).
Hyphenated HPLC-MS technique is an important method used for identifying complex mixtures, especially the phenolics in the crude extract or its fraction found in the plant, either by using standard compounds (target identification) or by comparing mass spectrum obtained with literatures (tentative identification) [ 6, 21, 40– 40].
CA was used as the target protein and the CLA was monitored by using standard aggregation assays, where heat induced aggregation of the target protein was recorded as light scattering at 360 nm.
The structure was refined in Crystallography and NMR System (CNS) by using standard simulated annealing protocols and the amplitude-based maximum likelihood target function [38] [39].
Another way to measure volatility is by using standard deviation.
Immunoblotting was performed by using standard methods.
CSF is processed by using standard methods.
Plugs were made by using standard techniques.
Target genes were amplified by PCR using standard techniques (Sambrook and Russell 2001), using primers listed in supplementary table S1, Supplementary Material online.
Expression levels of PcG target genes were determined by qRT-PCR using standard methods.
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